星空娱乐

杩戞棩锛屽寳浜悊宸ュぇ瀛﹂粍娓婁綑璇鹃缁勫湪siRNA鍔熻兘鍖栭暓绯荤撼绫虫潗鏂欐姉鑲跨槫娌荤枟鏂归潰鍙栧緱閲嶈鐮旂┒杩涘睍锛岀浉鍏虫垚鏋滀互鈥渟iRNA-functionalized lanthanide nanoparticle enables efficient endosomal escape and cancer treatment鈥濅负棰樺彂琛ㄥ湪Nano Research (涓闄竴鍖猴紝IF = 10.269)銆傝宸ヤ綔绗竴浣滆€呬负鍖椾含鐞嗗伐澶у鍓嶆部浜ゅ弶绉戝鐮旂┒闄/鐢熷懡瀛﹂櫌鐨勫崥澹悗浜庡┑濠靛拰纭曞＋鐢熸潕鍧わ紝閫氳浣滆€呬负鍥藉绾崇背绉戝涓績姊佸叴鏉扮爺绌跺憳銆佸寳浜悊宸ュぇ瀛﹂粍娓婁綑鏁欐巿銆佸箍瑗垮尰绉戝ぇ瀛﹁档姘哥ゥ鏁欐巿銆

闀х郴绾崇背鏉愭枡鍏锋湁鐙壒鐨勫厜瀛︺€佺數瀛﹀拰纾佸鎬ц川銆傜洰鍓嶏紝璁稿鐮旂┒鑱氱劍浜庨暓绯荤瀛愪笌鏍歌嫹閰/DNA鐨勭浉浜掍綔鐢紝浠ヨ璁″叿鏈夌嫭鐗规€ц兘鐨勬牳閰稿姛鑳界撼绫虫潗鏂欍€傛繁鍏ョ爺绌跺弻閾緎iRNA涓庨暓绯荤撼绫虫潗鏂欑殑浣滅敤鏈哄埗锛屾湁鍔╀簬寮€鍙戞柊鍨嬪皬骞叉壈鏍搁吀锛坰iRNA锛夐€掗€佷綋绯伙紝骞剁敤浜庣柧鐥呮不鐤椼€傚湪鏈鏂囦腑锛岃鍥㈤槦鐮旂┒浜唖iRNA涓庢棤閰嶄綋NaGdF₄绾崇背棰楃矑涔嬮棿鐨勭浉浜掍綔鐢ㄦ満鍒躲€傚湪涓€х幆澧冧笅锛宻iRNA纾烽吀楠ㄦ灦涓嶯aGdF₄绾崇背棰楃矑琛ㄩ潰鐨凣d閰嶄綅锛屽舰鎴恠iRNA/NaGdF₄鐞冨舰鏍搁吀绾崇背棰楃矑锛圫NA锛夛紝siRNA缁撳悎鍒扮撼绫虫潗鏂欒〃闈€傝繘鍏ョ粏鑳炲悗锛屽湪鍐呮兜浣撱€佹憾閰朵綋閰告€ф潯浠朵笅锛岀７閰稿熀鍥㈣川瀛愬寲锛岄檷浣巗iRNA瀵筃aGdF₄绾崇背棰楃矑鐨勪翰鍜屽姏锛宻iRNA涓嶯aGdF₄瑙ｇ锛堝浘1锛夈€傞€氳繃鑽у厜鍏辫仛鐒︽樉寰暅瑙傚療浜唖iRNA鑳炲唴閫冮€歌繃绋嬶紝鍚屾椂鍦ㄤ袱绉嶈嵎鐦ゅ皬榧犳ā鍨嬩笂鐮旂┒浜唖iRNA/NaGdF₄浣撶郴鍦ㄥ皬榧犱綋鍐呯殑鎶戠槫鏁堟灉銆

鍥1. NaGdF₄ NPs涓巗iRNA鐩镐簰浣滅敤浠ュ強siRNA鍔熻兘鍖朜aGdF₄ NPs浠庡唴娑典綋閫冮€哥殑杩囩▼绀烘剰鍥

鍥炬枃瀵艰锛

鍥2. siRNA涓嶯aGdF₄ NPs鐨勭浉浜掍綔鐢ㄣ€(a) NaGdF₄ NPs鐨凾EM鍥惧儚銆(b) siRNA/NaGdF₄ SNA浣撶郴鐨勫厓绱犲垎鏋愩€(c)鏃犻厤浣揘aGdF₄鍜宻iRNA/ NaGdF₄ SNA鐨勫昂瀵告暟鎹€(d)鐩镐簰浣滅敤鏈哄埗绀烘剰鍥俱€(e) siRNA/ NaGdF₄涓嬭繘琛屽嚌鑳剁數娉冲垎鏋愩€

鍥3. siRNA/NaGdF₄ SNA鐨勫唴娑典綋閫冮€歌繃绋嬨€(a)涓嶅悓杞煋鏃堕棿涓媠iRNA/NaGdF₄ SNA鍦–T26缁嗚優浜氱粏鑳炲畾浣嶇殑CLSM鍥惧儚锛屾瘮渚嬪昂涓25 渭m銆(b)鍜(c)骞冲潎鑽у厜寮哄害鍜孭earson鐩稿叧绯绘暟鐩稿簲浜庡浘a銆(d)浣跨敤姘柟鍜屽反鑿查湁绱燗1涓媠iRNA婧堕叾浣撻€冮€告儏鍐 (e) Pearson鐩稿叧绯绘暟鐩稿簲浜庡浘d銆(f)浣跨敤姘柟鎴栧反鑿查湁绱燗1鎶戝埗鍓傛椂PD-L1 mRNA鐨勭浉瀵硅〃杈俱€**** p < 0.0001銆

鍥4. siPD-L1/NaGdF4 SNA鍦4T1鍘熶綅涔宠吅鐧屾ā鍨嬩腑鐨勪綋鍐呮姉鑲跨槫鏁堟灉銆(a)瀹為獙杩囩▼鍜屽垎缁勪俊鎭€(b-d)鍦ㄧ粡杩嘝BS (G1)銆乻iNC/NaGdF4 SNA (G2)銆乻iPD-L1/NaGdF4 SNA (G3)娌荤枟鍚庯紝灏忛紶鑲跨槫缁勭粐鐨勫厜瀛︾収鐗(b)銆佽偪鐦ら噸閲(c)鍜岃偪鐦ょ敓闀挎洸绾(d)锛宯="""7銆(e)閫氳繃RT-qPCR妫€娴嬬殑鑲跨槫缁勭粐涓殑鐩稿PD-L1""" mRNA琛ㄨ揪銆(f)绗22澶╅噰闆嗙殑鑲虹粍缁囩収鐗囧拰瀵瑰簲鐨凥&E鏌撹壊鍒囩墖銆傞粍鑹插渾鍦堜唬琛ㄨ浆绉荤殑鑲跨槫缁撹妭锛涚粍缁囧垏鐗囩殑姣斾緥灏轰负100渭m銆(g)瀹為獙缁堢偣灏忛紶鐨勮娓呯敓鍖栨寚鏍囨娴嬬粨鏋溿€

璁烘枃閾炬帴锛歨ttps://link.springer.com/article/10.1007/s12274-022-4573-2

闄勪綔鑰呯畝浠嬶細

榛勬笂浣欙紝鍖椾含鐞嗗伐澶у鏁欐巿銆佸崥澹敓瀵煎笀锛涘叆閫夊浗瀹剁骇闈掑勾浜烘墠銆佸寳浜競绉戞妧鏂版槦銆傜爺绌跺唴瀹逛富瑕佸洿缁曟牳閰告妧鏈笌鑽墿/鐤嫍灞曞紑銆備互閫氳/绗竴浣滆€呭湪Sci Adv銆丼ignal Transduct Target Ther绛夊彂琛ㄨ鏂囩害60绡囷紝IF>10璁烘枃30浣欑瘒锛涘叡鍙戣〃璁烘枃绾90绡囷紝鎾板啓10浣欓儴涓撹憲绔犺妭锛岀敵璇16浠朵笓鍒┿€傛浘鑾峰緱骞胯タ绉戝鎶€鏈鑷劧绉戝涓€绛夊锛堢浜斿畬鎴愪汉锛夛紝涓編绾崇背鍖诲涓庣撼绫崇敓鐗╂妧鏈浼氣€滄湭鏉ヤ箣鏄熲€濆绛夎崳瑾夛紱鏄腑鍥界敓鐗╃墿鐞嗗浼氱撼绫崇敓鐗╁鍒嗕細绉樹功闀匡紝鍙﹀涓浼氱悊浜嬫垨濮斿憳锛涙槸Exploration鍓富缂栵紙Wiley鍑虹増锛夈€丆hinese Chemical Letters銆佺敓鐗╁寲瀛︿笌鐢熺墿鐗╃悊杩涘睍绛夊涓湡鍒(闈掑勾)缂栧銆

璇鹃缁勪富椤碉細https://www.x-mol.com/groups/Yuanyu_Huang-BIT